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在线回放|层析技术及过程分析技术介绍

生物制品圈  · 公众号  · 生物  · 2017-06-22 06:45

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讲者简介

speaker

Dr. Sebastijan Peljhan completed his PhD in theoretical physical chemistry at University of Ljubljana, Faculty of Chemistry and Chemical Technology and »Jožef Stefan« Institute, Ljubljana, Slovenia in 2012. He was awarded the »Maks Samec« award by University of Ljubljana for his outstanding contribution on atomistic modelling of corrosion processes, which were the research topic of his thesis. At the same time he joined application department of BIA Separations, where he was in charge of downstream processes and analytical method development of biomolecules on monolithic chromatographic supports. Currently, his main focus is development of process analytical technologies supported by advanced data manipulation used in upstream and downstream of biotechnological processes.

培训主题: introduction to monolith technology and PAT

part1: introduction

part2:convective interaction media monolithic columns

part3:plasmid purification on CIM monoliths

part4:HPLC frigerprinting approach for increased speed and flexibility of PAT

part5: applications

Case I: Separation of empty and full AAV capsid

Case II: AAV fermentation scale up

Case III: AAV fermentation progress

Case IV: AAV unit operations

Case V: Fingerprinting the Cohn I+II+III paste-batch to batch paste reproducibility

Case VI: MAb charge variant separation(displacement method)

Case VII: pDNA isolation & purification


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