Design and Perform Independent Research Experiments (90%)
Design and carry out experiments in C. elegans and mammalian cell systems (and potentially mice) using molecular biology (CRISPR-Cas9, DNA isolation, cloning and transfections, PCR, RNA isolation, RT-qPCR, RNAseq data analysis), biochemistry (protein analyses including isolation/purification, gel electrophoresis, western blotting, immunoprecipitation, proteomic data analysis), genetics (crosses, RNAi silencing, transgenic strain development and phenotypic analyses), and cell biology (fluorescence, confocal and high-content microscopy). Develop and troubleshoot new assays as needed, analyze data, prepare publication-quality figures, write manuscripts, keep meticulous records and perform any other duties, as needed. Participate in training sessions in aging research and present research findings at regional, national and international meetings (Keystone, Cold Spring Harbor and Gordon meetings).
Conduct training and administrative tasks associated with projects (10%)
Manage several projects and mentor undergraduate students, update inventories and maintain records for RNAi, plasmids, strains and primers. Coordinate the maintenance and purchase of laboratory equipment and supplies, maintain project organization including databases and manuscript development using OneDrive and Slack.
QUALIFICATIONS
Ph.D in Biochemistry or related fields
Publications in respected journals
Self-motivation, independence and drive
Excellent conceptual and analytical skills
Technical proficiency in Biochemistry and Molecular Biology
Superior communication skills and English writing abilities
Experience with C. elegans an asset, but not required
Experience with online bioinformatics platforms an asset, but not required.
